GB 15193.4-2014 Translated English of Chinese Standard (GB 15193.4-2014, GB15193.4-2014): National food safety standard -- Bacterial reversion mutation test [Buy--download True-PDF in 3-second: https://play.google.com/books > Sign in > This BOOK > "3-dots" > Export > Save as "*.pdf"]https://www.chinesestandard.net, 6. jun. 2024 - 23 sider Bacterial reverse mutation test includes Salmonella typhimurium reversion mutation test and Escherichia coli reversion mutation test. This Standard specifies the basic technical requirements for Salmonella typhimurium reversion mutation test. When selecting Escherichia coli for bacterial reversion mutation test, relevant literature shall be referred to. |
Indhold
Data processing and result evaluation | 16 |
Report | 17 |
Interpretation of the test | 18 |
Appendix A Mutant genes test types biological characteristics and spontaneous reversion of test strains | 19 |
Appendix B Standard diagnostic mutagens | 21 |
Positive mutagens recommended by OECD and USEPA | 23 |
Almindelige termer og sætninger
Add distilled water aminofluorene ampicillin solution auxotroph bacterial liquid Bacterial reversion mutation biotin bottom culture medium broth agar medium culture medium plate Daunorubicin Dexon diagnostic mutagens dissolve DMSO Escherichia coli filter paper flat to solidify Frameshift mutation frameshift mutation Base Glucose-6-phosphate sodium salt Heat and melt histidine hydrogen phosphate incubate at 37 inh inh inh inoculation loop lay it flat Lipopolysaccharide Magnesium sulfate master plate Methyl methanesulfonate Mitomycin mL of bottom mmol mol/L MPa for 20 number of reversion nutrient broth agar phenobarbital phenylenediamine pipette plate incorporation method potassium chloride reagents Result determination Result evaluation reversion colonies reversion mutation test S9 mixture Salmonella typhimurium Sodium azide solubility solution 0.5 mmol/L solvent control group spontaneous reversion spot test method standard mutagen sterilize at 0.103 strain identification test strains Strain test substance test tubes test types top agar top culture medium toxicity tryptophan untreated control group Vaccination water bath WP2uvrA µL/dish